Journal: Journal of Traditional and Complementary Medicine

Article Title: Antibacterial oral sprays from kaffir lime ( Citrus hystrix DC.) fruit peel oil and leaf oil and their activities against respiratory tract pathogens

doi: 10.1016/j.jtcme.2019.09.003

Figure Lengend Snippet: Antibacterial activity of three Kaffir lime fruit peel oil oral sprays and three Kaffir lime leaf oil oral sprays against one Group A streptococcal clinical isolate and 3 standard strains of respiratory tract pathogenic bacteria, using broth microdilution method.

Article Snippet: All oral sprays exhibited antibacterial activity against a Group A streptococcal clinical isolate and three respiratory pathogens ( S. aureus ATCC 29213, S. pneumoniae ATCC 49619, and H. influenza e ATCC 49247), with the strongest activity against H. influenza e ATCC 49247.

Techniques: Activity Assay, Bacteria

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Genetically Engineered Lipopeptide Antibiotics Related to A54145 and Daptomycin with Improved Properties ▿ †

doi: 10.1128/AAC.01307-09

Figure Lengend Snippet: Strains and plasmids

Article Snippet: Strain or plasmid Relevant characteristic(s) Source or reference Strains E. coli BAS849 Δ lamB106 imp-4213 39 BW25113 lacl q rrnB T14 Δ lacZ WJ16 hsdR514 13 ML22 DH10B::pUZ8002 11 E. faecalis 201 CLSI test strain ATCC 49452 312 E. faecalis 201 mutant with reduced susceptibility to Dap Cubist strain collection E. faecium 14 CLSI test strain ATCC 6569 384 E. faecium 14 mutant with reduced susceptibility to Dap Cubist strain collection S. aureus 42 CLSI test strain ATCC 29213 339 CLSI clinical MRSA isolate ATCC 43300 MW2 MRSA; genomic sequence available 2 1616 MW2 with reduced susceptibility to Dap 17 1695 MW2 with reduced susceptibility to Dap 17 S. pneumoniae 402 CLSI test strain ATCC 6303 S. fradiae XH25 rpsL ; producer of A54145 Alexander et al., submitted DA613 XH25 Δ lptI :: tsr Alexander et al., submitted DA740 XH25 Δ lptBCD Alexander et al., submitted DA901 XH25 Δ lptBCD Δ lptI :: tsr Alexander et al., submitted DA1187 XH25 Δ lptEFABCDGHJKLMNPI Alexander et al., submitted XH1000 DA1187::pKN55 This study KN707 DA901::pKN56 This study KN681 DA740::pKN56 This study KN715 DA901::pKN57 This study KN689 DA740::pKN57 This study KN723 DA901::pKN58 This study KN697 DA740::pKN58 This study KN728 DA901::pKN59 This study KN701 DA740::pKN59 This study KN730 DA901::pKN60 This study KN705 DA740::pKN60 This study KN649 DA1187::pKN61 This study KN665 XH1000::pKN61 This study KN661 DA1187::pKN63 This study KN677 XH1000::pKN63 This study XH1003 DA1187::pXH11 This study XH1035 XH1000::pXH11 This study Plasmids pDA2048 pECBAC1-derived; oriT att/int φC31 Am r :: ermEp*lptBCDGH Alexander et al., submitted pDA2054 pECBAC1-derived; oriT att/int φC31 Am r :: lptEFABCDGHJKLMNP Alexander et al., submitted pRT802 Kan r att/int φBT1 19 pKN54 pRT802:: ermEp * This study pKN55 pKN54:: dptIJ This study pKN56 pDA2048 Δ D-Lys8-CAT :: D-Ala-CAT This study pKN57 pDA2048 Δ D-Lys8-CAT :: D-Ser-CAT This study pKN58 pDA2048 Δ D-Lys8-CAT :: D-Asn-CAT This study pKN59 pDA2048 Δ D-Asn11-CAT :: D-Ala-CAT This study pKN60 pDA2048 Δ D-Asn11-CAT :: D-Ser-CAT This study pKN61 pDA2054 Δ D-Glu2-D-Lys8 :: D-Asn2-D-Ala8 This study pKN63 pDA2054 Δ D-Glu2-Thr4 :: D-Asn2-D-Thr4 This study pXH11 pDA2054 Δ D-Glu2-CAT :: D-Asn2-CAT This study Open in a separate window Strains and plasmids.

Techniques: Plasmid Preparation, Mutagenesis, Sequencing

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Genetically Engineered Lipopeptide Antibiotics Related to A54145 and Daptomycin with Improved Properties ▿ †

doi: 10.1128/AAC.01307-09

Figure Lengend Snippet: Antibacterial spectra of hybrid lipopeptides

Article Snippet: Strain or plasmid Relevant characteristic(s) Source or reference Strains E. coli BAS849 Δ lamB106 imp-4213 39 BW25113 lacl q rrnB T14 Δ lacZ WJ16 hsdR514 13 ML22 DH10B::pUZ8002 11 E. faecalis 201 CLSI test strain ATCC 49452 312 E. faecalis 201 mutant with reduced susceptibility to Dap Cubist strain collection E. faecium 14 CLSI test strain ATCC 6569 384 E. faecium 14 mutant with reduced susceptibility to Dap Cubist strain collection S. aureus 42 CLSI test strain ATCC 29213 339 CLSI clinical MRSA isolate ATCC 43300 MW2 MRSA; genomic sequence available 2 1616 MW2 with reduced susceptibility to Dap 17 1695 MW2 with reduced susceptibility to Dap 17 S. pneumoniae 402 CLSI test strain ATCC 6303 S. fradiae XH25 rpsL ; producer of A54145 Alexander et al., submitted DA613 XH25 Δ lptI :: tsr Alexander et al., submitted DA740 XH25 Δ lptBCD Alexander et al., submitted DA901 XH25 Δ lptBCD Δ lptI :: tsr Alexander et al., submitted DA1187 XH25 Δ lptEFABCDGHJKLMNPI Alexander et al., submitted XH1000 DA1187::pKN55 This study KN707 DA901::pKN56 This study KN681 DA740::pKN56 This study KN715 DA901::pKN57 This study KN689 DA740::pKN57 This study KN723 DA901::pKN58 This study KN697 DA740::pKN58 This study KN728 DA901::pKN59 This study KN701 DA740::pKN59 This study KN730 DA901::pKN60 This study KN705 DA740::pKN60 This study KN649 DA1187::pKN61 This study KN665 XH1000::pKN61 This study KN661 DA1187::pKN63 This study KN677 XH1000::pKN63 This study XH1003 DA1187::pXH11 This study XH1035 XH1000::pXH11 This study Plasmids pDA2048 pECBAC1-derived; oriT att/int φC31 Am r :: ermEp*lptBCDGH Alexander et al., submitted pDA2054 pECBAC1-derived; oriT att/int φC31 Am r :: lptEFABCDGHJKLMNP Alexander et al., submitted pRT802 Kan r att/int φBT1 19 pKN54 pRT802:: ermEp * This study pKN55 pKN54:: dptIJ This study pKN56 pDA2048 Δ D-Lys8-CAT :: D-Ala-CAT This study pKN57 pDA2048 Δ D-Lys8-CAT :: D-Ser-CAT This study pKN58 pDA2048 Δ D-Lys8-CAT :: D-Asn-CAT This study pKN59 pDA2048 Δ D-Asn11-CAT :: D-Ala-CAT This study pKN60 pDA2048 Δ D-Asn11-CAT :: D-Ser-CAT This study pKN61 pDA2054 Δ D-Glu2-D-Lys8 :: D-Asn2-D-Ala8 This study pKN63 pDA2054 Δ D-Glu2-Thr4 :: D-Asn2-D-Thr4 This study pXH11 pDA2054 Δ D-Glu2-CAT :: D-Asn2-CAT This study Open in a separate window Strains and plasmids.

Techniques:

Journal: Germs

Article Title: Detection of antibiotic-producing Actinobacteria in the sediment and water of Ma’in thermal springs (Jordan)

doi: 10.18683/germs.2018.1146

Figure Lengend Snippet: Diameter of inhibition zones (mm) ± SD formed around the crude extract obtained from antibacterial cultures

Article Snippet: In respect to strain M3-2, it was found to be active against S. aureus ATCC 29213 (14 mm) and B. cereus ATCC 11778 (9 mm) – . table ft1 table-wrap mode="anchored" t5 caption a7 M1-1 M2-2 M3-2 P. aeruginosa ATCC 2785 9 ± 0.58 NA NA E. coli ATCC 25922 NA 9 ± 2.3 NA S. aureus ATCC 29213 NA 12 ± 2 14 ± 2 B. cereus ATCC 11778 NA 11 ± 1.5 9 ± 1 Open in a separate window caption a8 Diameter of inhibition zones (mm) ± SD formed around the crude extract obtained from antibacterial cultures Actinobacterial diversity in water samples Actinobacteria from water samples could not be isolated by applying the same methods used for sediment samples.

Techniques: Inhibition